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. 2015 Jun 2;21(6):845–854. doi: 10.1016/j.cmet.2015.04.016

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Molecular Analysis of Cox15^sm/sm::Opa1^tg Skeletal Muscle

(A) Western blot analysis of OPA1, SDHA (CII), ATP5A (CV), UQCRC2 (CIII), NDUFA9 (CI), COX1, and COX4 (CIV); GAPDH is taken as a loading reference.

(B) Densitometric analysis of MRC subunits. Solid blue: WT (n = 4); blue outline: Opa1^tg (n = 4); solid red: Cox15^sm/sm (n = 4); red outline: Cox15^sm/sm::Opa1^tg (n = 4). Error bars represent SEM. The asterisks represent the significance levels calculate by unpaired, two-tail Student’s t test. ^∗∗p < 0.01; ^∗∗∗p < 0.005.

(C) mRNA expression analysis of CIV- and mitochondrial-biogenesis-related genes. Gene transcripts, retrotranscribed into cDNA, were quantified by RT-PCR; normalized to the amount of the β-Actin gene transcript; taken as a standard; and expressed as time-fold variation relative to the WT. Error bars represent SEM. The asterisks represent the significance levels calculate by unpaired, two-tail Student’s t test. ^∗∗p < 0.01; ^∗∗∗p < 0.005.