Fig 5. Hypergravity regulated OPN expression by promoting Runx2 expression.
(A) Runx2 siRNA downregulated both Runx2 and OPN expression. (B and C) Statistical analyses of results in A (n = 6). (D) Western blotting results of OPN in culture medium after Runx2 siRNA transfection. (E) Statistical analyses of results in I (n = 6). n.s. indicates no statistical difference between 1 ×g and 20 ×g. *, statistically significant difference (P < 0.05).