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. 2015 Jun 5;11(6):e1005278. doi: 10.1371/journal.pgen.1005278

Fig 3. The effect of RecX proteins on ATPase activity of RecA variant proteins.

Fig 3

(A) Effects of EcRecX protein. Reactions contained 3 μM RecA variant, 5 μM M13mp18 ssDNA, 0.5 μM SSB, and 3 mM ATP. The ATP hydrolysis by RecA protein was initiated by the addition of ATP and SSB protein. After 7 min, 100 nM of RecX protein was added and hydrolysis reaction was monitored as shown above. (B) The effect of NgRecX protein on ATPase activity of WT RecA and RecA V79L variant proteins. Reactions were carried out as in panel A, with NgRecX substituted for EcRecX protein where noted.