Fig 5. The effects of RecA variants on UV radiation and ciprofloxacin sensitivity.

(A) Mutant strains expressing wild type RecA or the indicated RecA variant proteins were plated and exposed to UV radiation as described in Materials and Methods. The colonies were counted to obtain viability data, which was normalized against the zero dose point to obtain percent survival. (B) Cells were grown to log phase, serially diluted 1:10, and spot plated on LB plates either with or without 0.01 μg/ml ciprofloxacin as indicated.