Table 1. Effect of EcRecX on RecA and RecA variant filaments.
| Total molecules counted | Full filaments (%) | Medium filaments (%) | Small filaments (%) | Very small filaments (%) | SSB/DNA (%) | Linear (%) | |
|---|---|---|---|---|---|---|---|
| WT | 1382 | 87.8 | 1.7 | 0.4 | 0 | 0 | 10.1 |
| WT + EcRecX | 3653 | 1.3 | 10.3 | 52.0 | 30.2 | 3.8 | 2.4 |
| I102L | 1458 | 87.2 | 1.7 | 0.4 | 0 | 0 | 10.7 |
| I102L + EcRecX | 1626 | 58.3 | 13.2 | 10.4 | 7.0 | 0.7 | 10.4 |
| V79L | 1147 | 90.6 | 0.2 | 0 | 0 | 0 | 9.1 |
| V79L + EcRecX | 1056 | 86.9 | 1.7 | 0 | 0 | 0 | 11.4 |
| E86G/C90G | 1125 | 88.4 | 0 | 0 | 0 | 0 | 11.6 |
| E86G/C90G + EcRecX | 1157 | 64.4 | 19.0 | 6.1 | 0.6 | 0 | 10.0 |
Quantitation of electron microscopy results. The fraction of observed molecules in each category described in the text and in Fig 4 are summarized.