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. 2015 Jun 4;96(6):883–893. doi: 10.1016/j.ajhg.2015.04.010

Figure 3.

Figure 3

Knockdown of the Human COL6A3 Exon 41 Ortholog in Zebrafish Embryos Causes Motor Neuron Pathfinding Errors

(A–F) Representative lateral views of 2 days post fertilization zebrafish embryos stained with acetylated tubulin. In control embryos (A), motor neurons have completed migration toward the myotome. In MO1-injected embryos (B–D), secondary axons exit the periphery but fail to migrate along the common path. This phenotype is concentration dependent: class I corresponding to one motor neuron abnormality (B); class II to two or three (C); and class III to four or more motor neuron abnormalities (D). Embryos injected with MO2 (E) and MO3 (F) do not present pathfinding errors but develop a body curvature defect reminiscent of collagen deposition mutants previously described.16

(G) Percentage of affected embryos under the conditions being evaluated above. ∗∗∗p < 0.0001; ns = nonsignificant.