Fig. 2.

hAECs inhibit upregulation of T cell activation markers and retain T cells in a naïve phenotype. Naïve CD4+ T cells were stimulated with anti-CD3/CD28 in the presence or absence of hAECs for 5 days and then analyzed by flow cytometry. a, b Representative profiles of CD25 expression on stimulated CD4+ T cells. c, d Representative profiles of CD44 and CD62L expression on stimulated CD4 + CD25+ T cells. CD62LhiCD44lo denotes naïve T cells, CD62LloCD44hi denotes memory cells. Data are representative of two independent experiments with n = 3 mice