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. 2015 Jun 7;15:457. doi: 10.1186/s12885-015-1456-x

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© VanKlompenberg et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Gene expression of ATP-dependent binding cassette transporters. a Microarray analysis of mammary glands from Apc^Min/+ and Apc^+/+ mice at d16 of lactation show a decrease in ABCG2 and increase in MDR1 expression due to Apc mutation. b MDR1 gene expression in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after 24 h treatment with either solvent control, paclitaxel, cisplatin or doxorubicin. MDR1 expression was significantly increased in cells from MMTV-PyMT;Apc^Min/+ mice after treatment with paclitaxel and doxorubicin but not cisplatin. c ABCG2 gene expression in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after treatment for 24 h with either solvent control, paclitaxel, cisplatin or doxorubicin. ABCG2 expression was not different between MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ cells and chemotherapy treatment had no effect on ABCG2 expression. d Representative western blots for MDR1 and ABCG2 in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after treatment for 24 h with either solvent control, paclitaxel, cisplatin or doxorubicin. e Quantification of MDR1 western blots shows that MMTV-PyMT;Apc^Min/+ cells have enhanced MDR1 expression when treated with doxorubicin. f Quantification of ABCG2 western blots shows that MMTV-PyMT;Apc^+/+ cells have elevated ABCG2 protein expression compared to MMTV-PyMT;Apc^Min/+ cells. Results in B, C, E and F are shown as the means ± SEM from 3 independent experiments; *P < 0.05 when comparing MMTV-PyMT;Apc^Min/+ to MMTV-PyMT;Apc^+/+ cells and #P < 0.05 when comparing MMTV-PyMT;Apc^Min/+ cells treated with solvent control or chemotherapy agent.