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. 2015 May 12;13:156. doi: 10.1186/s12967-015-0512-2

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© Crisostomo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cytokines levels in pericardial fluid. Cytokines levels were measured in the filtered pericardial fluid using quantitative ELISA kits for porcine TNF-alpha and active TGF-beta. Absorbance was read at 450 nm and cytokines levels (expressed in pg/ml) were extrapolated from a calibration curve. The graphs represent mean values ± standard deviation of four independently performed experiments (n = 4) for TNF-alpha (a) and active TGF-beta (b) determinations. The results were analyzed through a Student’s t-test for variables with parametric distribution, comparing each measure with the control group (*p < .05)