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. 2015 May 28;14:73. doi: 10.1186/s12934-015-0252-2

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© Gold et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Total specific carbon measured for metabolites affected by engineering of localized pathway and global metabolism. Estimated using a single 2-parameter exponential rise to maximum curve fit in SigmaPlot11.0 and values taken at 48 h. Referring always to strain TY920, strains TY757 and TY985 were evaluated downstream of DHS inclusively, strain TY952 downstream of prephenate/phenylpyruvate inclusively, and TYR1 and TYRC strains, grown with or without methionine, downstream of 4HPP inclusively