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. 2015 Apr 22;6(2):132–143. doi: 10.1002/jcsm.12023

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© 2015 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of the Society of Sarcopenia, Cachexia and Wasting Disorders

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Ghrelin improves cisplatin-induced myotubes breakdown. (A) Immunofluorescence staining for anti-myosin/myosin heavy chain (MHC) antibody in C2C12 myotubes. MHC staining outlines the myotubes (red). 4'6-Diamidino-2-phenylindole was used to stain the nuclei (blue). (B) Myotubes size expressed as % from vehicle. (C–D) Western blot of MHC and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in C2C12 myotubes. V, vehicle-treated group; C, cisplatin-treated group; CG, cisplatin + ghrelin-treated group.