Skip to main content
. 2015 Apr 2;523(11):1639–1663. doi: 10.1002/cne.23761

Figure 1.

Figure 1

rVSV(VSV‐G) transmission shows an anterograde, and rVSV(RABV‐G) shows a retrograde, polysynaptic pattern of transmission among neurons in mice. (A–H) rVSV(VSV‐G) was injected into the dorsal striatum (DS) (A–D) or primary motor cortex (E–H), and animals were sacrificed 3 dpi. (A) Schematic of regions expected to be labeled by anterograde transsynaptic transmission (blue) of a virus from a DS injection (injection needle, green). (B–D) At 3 dpi, DS injections of rVSV(VSV‐G) resulted in patterns of infection consistent with anterograde transsynaptic transmission. Infected cells were observed near the injection site in the DS (white arrowhead), as well as in the GPe (red arrowhead), SNr (pink arrowhead), and the thalamus. The cortex, which projects to the DS, was not labeled (brown arrowhead). Higher‐magnification images of neurons in the DS (C) and GPe (D) are shown. (E) Schematic of regions expected to be labeled by anterograde transsynaptic transmission (blue) of a virus from a primary motor cortex injection (injection needle, green). (F–H) Injection of rVSV(VSV‐G) into the primary motor cortex labeled cells locally in the cortex (brown arrowhead), and the same regions as a direct DS injection, including the DS (white arrowhead), GPe (red arrowhead), STN (yellow arrowhead), SNr (pink arrowhead), and thalamus (purple arrowhead). High‐magnification images of DS neurons (G), and neurons in the GPe (H) are provided. (I) Schematic of regions expected to be labeled by initial infection by retrograde uptake, and/or by retrograde transsynaptic transmission (orange), with the nucleus basalis (NB) predicted to be labeled only by retrograde transmission (magenta) of rVSV(RABV‐G) from a DS injection (injection needle, green). (J–L) rVSV(RABV‐G) was injected into the DS. At 3 dpi, this injection resulted in infected cells in retrograde targets, including local infection in the DS (white arrowhead), cortex (brown arrowhead), NB (red arrowhead), and the thalamus (purple arrowhead). High magnifications of a cortical neuron (K), and NB neurons (L) are shown. (M) Schematic of regions expected to be labeled by initial infection by retrograde uptake, and/or by transsynaptic transmission (orange) of a retrograde virus from a primary motor cortex injection (injection needle, green). (N–P) Injections of rVSV(RABV‐G) into the primary motor cortex labeled neurons at the injection site in the cortex (brown arrowhead), as well as the NB (red arrowhead) and thalamus (purple arrowhead), but not the DS. Higher magnification of a cortical neuron (O) and thalamic neurons (P) are shown. Multiple types of neurons, including glutamatergic (e.g., cortical pyramidal neurons, panels K,O), GABAergic (DS medium spiny neurons, panels C,G), and cholinergic (NB neurons, panel L), were labeled. DS = dorsal striatum, Th = thalamus, STN = subthalamic nucleus, GPe = globus pallidus external segment, SNr = substantia nigra pars reticulata, NB = nucleus basalis. Scale bars = 1 mm in B,F,J,N; 50 μm in C,D,G,H,K,L,O,P.