. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: J Pharmacol Toxicol Methods. 2015 Feb 12;73:1–6. doi: 10.1016/j.vascn.2015.02.001

Table 1.

Linearity of the LDH assay

tBHP (μM)	Slope	R²	y-intercept at x = 0	n (x), n (y)
0	0.6634 ± 0.0013	0.9996	0.1275 ± 0.0023	91, 8
30	0.6769 ± 0.0014	0.9996	0.1317 ± 0.0025	91, 8
60	0.7129 ± 0.0017	0.9995	0.1369 ± 0.0030	91, 8
90	0.7634 ± 0.0022	0.9993	0.1464 ± 0.0038	91, 8
120	0.8417 ± 0.0028	0.9990	0.1604 ± 0.0049	91, 8
200	0.9848 ± 0.0058	0.9969	0.2064 ± 0.0100	91, 8

The linearity of the LDH assay was determined by linear regression analysis of the A₄₉₀ absorbance values obtained during the first three hours of the assay at measurement intervals of 2 min. The assay showed a highly linear response (R² > 0.99) throughout all conditions.