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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Neonatology. 2015 Mar 21;107(4):304–311. doi: 10.1159/000371835

Figure 4. CXCR4 Blockade Increases Lung Vascular Density in HILI.

Figure 4

A. Lung sections stained with vWF, red and 6-diamidino-2-phenylindole (DAPI; blue) demonstrating improved capillary density in hyperoxia exposed rats treated with AMD 3100. Original magnification x 100, scale bars: 100 μm.

B. Increased vascular density in hyperoxic-AMD3100 rats (*p<0.0001; RA-PL vs. hyperoxia-PL; **p<0.05; hyperoxia-PL vs. hyperoxia-AMD3100; n=10/group). White bars represent RA animals and black bars represent hyperoxia animals.

C. Increased lung VEGF concentration in hyperoxic-AMD3100 rats (*p<0.05; RA-PL vs. hyperoxia-PL; **p<0.0001; hyperoxia-PL vs. hyperoxia-AMD3100; n=5/group).

D. Increased lung VEGFR2 expression in hyperoxic-AMD3100 rats (*p<0.004; RA-PL vs. hyperoxia-PL; **p<0.02; hyperoxia-PL vs. hyperoxia-AMD3100; n=5/group). RA is room air and HYP is hyperoxia. VEGFR2 expression is normalized to β-actin.

E. Increased RVSP in hyperoxia groups (*p<0.05; RA-PL/AMD3100 vs. hyperoxia-PL/AMD3100; n=10/group). There was no difference in the RVSP between hyperoxia groups.

F. Increased RV/LV+S in hyperoxia groups (*p<0.05; RA-PL/AMD3100 vs. hyperoxia-PL/AMD3100; n=10/group). There was no difference in the RV/LV+S between hyperoxia groups.

G. Increased MWT in hyperoxia groups (*p<0.05; RA-PL/AMD3100 vs. hyperoxia-PL/AMD3100; n=10/group). There was no difference in the MWT between hyperoxia groups.