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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Plant Biol (Stuttg). 2014 Jul 2;17(0 1):59–65. doi: 10.1111/plb.12215

Figure 1. L. gibba pre-miR166a as backbone for amiRNA expression.

Figure 1

Secondary structure of the miRNA precursor Lg-miR166, predicted by RNAfold (ViennaRNA package 2). The sequence corresponding to miRNA/*miRNA duplex is colored in purple (A). Comparison between secondary structures of the endogenous and the modified precursor (B). Predicted binding site of amiRNA to the CH42 mRNA. Circle indicates G:U wobble pairing (C). Schematic diagram of the construct designed to express the amiRNA precursor. The construct contains a selectable marker that confers resistance to PPT as well as an independent GFP expression cassette to track regeneration of the callus (D). Transcription of the amiRNA precursor is driven by the CaMV 35S promoter.