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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Plant Biol (Stuttg). 2014 Jul 2;17(0 1):59–65. doi: 10.1111/plb.12215

Figure 2. Stable transformation and regeneration of L. minor callus with a GFP expression construct.

Figure 2

Green fluorescence signal detected at various stages of selection in liquid media (A, B, C). 5 weeks after transformation transgenic fronds began to regenerate from callus (D). Transformed fronds expressing GFP were regenerated with no phenotypic abnormalities (E), and the transgenes were stably maintained through generations (F). day = days after transformation; wat = weeks after transformation.