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Western blot of B. pertussis isolates reveals deficiency in possible acellular pertussis vaccine protein components. (a) Prn, 69 kDa; (b) Fim3, 22.5 kDa; and (c) Fim2, 22 kDa. T—Bp Tohama I; Bp CA2010 isolates: 2, 4, 5, 9, 11, and 12. Recombinant (r) proteins were used as western blot controls, and all blue or dual color protein molecular weight markers for size standards. The black box highlights the protein of interest.
