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CypA was confirmed to interact with VP4 in yeast. The bait plasmid pGB-VP4 and the prey plasmid pGA-CypA isolated from positive colonies were cotransformed into yeast Y2H strains and retested on DDO, QDO, and QDO/X/A. A positive control (pGBKT7-53 and pGADT7-T, +), negative control (pGBKT7-Lam and pGADT7-T, -Lam), and blank control (pGBKT7 and pGADT7, −) were also included.
