VP4-U |
AGAGAATTCGCCGACAAGGGGTACGAG |
EcoR I |
pGB-VP4 |
VP4-L |
AAACTGCAGAGCCATGGCAAGGTGGTA |
Pst I |
|
HA-VP4-U |
CTGAATTCGCCGACAAGGGGTACGAG |
EcoR I |
pCAH-VP4 |
HA-VP4-L |
GCCTCGAGAGCCATGGCAAGGTGGTA |
Xho I |
|
CypA-U |
AAAGAATTCATGGCCAACCCCGTCGTG |
EcoR I |
pCAF-CypA |
CypA-L |
ATACTCGAGCCGAGAGCTGCCCGCAGTT |
Xho I |
|
pGEX-CYPA-U |
GGCGAATTCGCCAACCCCGTCGTGTTC |
EcoR I |
pGEX-CypA |
pGEX-CYPA-L |
GCTGAGCTCCGAGAGCTGCCCGCAGTT |
Xho I |
|
T7SPU |
TAATACGACTCACTATAGGGC |
|
pGADT7 |
3ASPL |
ACACGTAGCACGTGGTAGA |
|
|
FVP5 |
CCTTCTGATGCCAACAAC |
|
Real-time RT-PCR |
RVP5 |
ACAATTAGCCCTGACCCT |
|
|
PVP5 |
FAM-CGGACGACACCCTGGAGAAGCA-BHQ1 |
|
|
RNA#1 |
Sense, 5′-CCGAGUGGUUGGACGGCAATT-3′ |
|
siRNAs |
Antisense, 5′-UUGCCGUCCAACCACUCGGTT-3′ |
|
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RNA#2 |
Sense, 5′-ACGGCAAGACGAGCAAGCATT-3′ |
|
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Antisense, 5′-UGCUUGCUCGUCUUGCCGUTT-3′ |
|
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RNA#3 |
Sense, 5′-GACGAGAACUUCAUCCUGATT-3′ |
|
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Antisense, 5′-UCAGGAUGAAGUUCUCGUCTT-3′ |
|
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Negative control (NC) |
Sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ |
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Antisense, 5′-ACGUGACACGUUCGGAGAATT-3′ |
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