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. 2015 May 24;2015:719454. doi: 10.1155/2015/719454

Table 1.

Primers and siRNAs used in this study.

Primers/siRNAs Sequences (5′-3′) Restriction enzyme Usage
VP4-U AGAGAATTCGCCGACAAGGGGTACGAG EcoR I pGB-VP4
VP4-L AAACTGCAGAGCCATGGCAAGGTGGTA Pst I
HA-VP4-U CTGAATTCGCCGACAAGGGGTACGAG EcoR I pCAH-VP4
HA-VP4-L GCCTCGAGAGCCATGGCAAGGTGGTA Xho I
CypA-U AAAGAATTCATGGCCAACCCCGTCGTG EcoR I pCAF-CypA
CypA-L ATACTCGAGCCGAGAGCTGCCCGCAGTT Xho I
pGEX-CYPA-U GGCGAATTCGCCAACCCCGTCGTGTTC EcoR I pGEX-CypA
pGEX-CYPA-L GCTGAGCTCCGAGAGCTGCCCGCAGTT Xho I
T7SPU TAATACGACTCACTATAGGGC pGADT7
3ASPL ACACGTAGCACGTGGTAGA
FVP5 CCTTCTGATGCCAACAAC Real-time RT-PCR
RVP5 ACAATTAGCCCTGACCCT
PVP5 FAM-CGGACGACACCCTGGAGAAGCA-BHQ1
RNA#1 Sense, 5′-CCGAGUGGUUGGACGGCAATT-3′ siRNAs
Antisense, 5′-UUGCCGUCCAACCACUCGGTT-3′
RNA#2 Sense, 5′-ACGGCAAGACGAGCAAGCATT-3′
Antisense, 5′-UGCUUGCUCGUCUUGCCGUTT-3′
RNA#3 Sense, 5′-GACGAGAACUUCAUCCUGATT-3′
Antisense, 5′-UCAGGAUGAAGUUCUCGUCTT-3′
Negative control (NC) Sense, 5′-UUCUCCGAACGUGUCACGUTT-3′
Antisense, 5′-ACGUGACACGUUCGGAGAATT-3′