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Effects of inhibitors of phosphoinositide-3-kinase on phospholipase D activity and glucose metabolism in insulin-stimulated hepatocytes. (a) Phospholipase D activity, (b) uptake of 2-D-[3H]glucose and incorporation of D-[U 14C]glucose into glycogen. Effect of insulin on PLD activity and glucose metabolism in the wortmannin- or LY294002-treated hepatocytes was determined as described in the Methods. ∗ p < 0.05, insulin versus control.
