Figure 6.
Effect of halopemide and ceramide on phospholipase D activity and glucose metabolism stimulation by insulin in the isolated hepatocytes. (a) Phospholipase D activity, (b) uptake of 2-D-[3H]glucose, (c) incorporation of D-[U 14C]glucose into glycogen. Effect of insulin on phospholipase D activity and glucose metabolism in the halopemide or C6-ceramide-pretreated hepatocytes was determined as described in the Methods. ∗ p < 0.05, insulin versus control; ∗∗ p < 0.05, insulin + drug versus insulin; ∗∗∗ p < 0.05, C6-ceramide in basal condition versus basal condition, control cells.