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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: DNA Repair (Amst). 2015 May 14;31:73–79. doi: 10.1016/j.dnarep.2015.05.005

Figure 4. MCF10A cells expressing R280H hXRCC1 accumulate double strand breaks in the G1 cell cycle phase.

Figure 4

MCF10A pools expressing either WT or R280H hXRCC1 were treated with (A) 30 mM H2O2 for 30 min on ice and allowed to recover for 1, 2, and 6 hours or (B) 2mM MMS for 2 hours at 37°C and allowed to recover for 1, 2, and 4 hours. Cells were stained with FITC-conjugated γH2AX antibody and propidium iodide to assess the levels of double-strand breaks and the cell cycle phase, respectively, and analyzed by flow cytometry. Data are plotted as mean ± S.E. ** denotes p < 0.01.