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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: DNA Repair (Amst). 2015 May 14;31:73–79. doi: 10.1016/j.dnarep.2015.05.005

Figure 5. Expression of R280H hXRCC1 in MCF10A cells results in an increased level of double strand breaks.

Figure 5

Representative γH2AX/53BP1 immunostaining images of MCF10A expressing WT (A.) or R280H hXRCC1 (B.). Left upper panel represents γH2AX staining (red), right upper - 53BP1 staining (green), right bottom - DAPI staining (blue), and left bottom is a composite picture of all three panels (yellow for co-localizing foci). C. Only co-localizing foci (yellow on composite picture) were counted in WT or R280H hXRCC1 expressing cells. A total of at least 30 cells were scored for each cell line. Data are plotted as mean ± S.E. ** denotes p < 0.01.