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. Author manuscript; available in PMC: 2015 Jun 7.
Published in final edited form as: J Cell Physiol. 2011 Jun;226(6):1702–1712. doi: 10.1002/jcp.22501

Fig. 6.

Fig. 6

Effect on pHi of overexpression of NHE6 or NHE1 knockdown in MG63 cells. MG63 cells grown on glass were transfected with either control vector, NHE6-expressing plasmid, control siRNA, or NHE1 siRNA. Forty-eight hours later, a glass chip was loaded with BCECF and equilibrated in standard solution. Standard solution, Na+-free solution, and acetic acid load solution are the same as in previous figures. A: Effect of NHE6 over expression on Na+ withdrawal in MG63 cells. Black trace: control vector transfected MG63 cells were exposed to Na+-free solution from 90 to 355 sec then transferred back into standard solution. Grey trace: MG63 cells transfected with NHE6-expressing plasmids were exposed to Na+-free solution from 210 to 380 sec then transferred back in to standard solution. Horizontal bars indicate periods of Na+ withdrawal. B: Effect of NHE6 over expression on acetic acid load in MG63 cells. Black trace: control vector transfected MG63 cells were exposed to acetic acid loads olution from 210 to 610 sec then transferred back in to standard solution. Grey trace: MG63 cells transfected with NHE6-expressing plasmids were exposed to acetic acid load from 185to 835 sec then transferred back into standard solution. Horizontal bars indicate periods of acetic acid loading. C: Real-time PCR analysis of NHE6 expression in MG63 cells transfected with control vector or NHE6-expressing plasmid. Results are presented as percentage of GAPDH level. Note that the difference is several hundred fold; the Y-axis is log scale. D: Effect of NHE1 knockdown on acetic acid load in MG63 cells. Black trace: control siRNA transfected MG63 cells were exposed to acetic acid load solution from 105 to 380 sec then transferred back into standard solution. Grey trace: MG63 cells transfected with NHE6-expressing plasmids were exposed to acetic acid load from 105 to 565 sec then transferred back into standard solution. Horizontal bars indicate periods of acetic acid loading. E: Real-time PCR analysis of NHE1 expression in MG63 cells transfected with control siRNA or NHE1 siRNA. Results are presented as percentage of GAPDH level.