Fig. 2. Deficiency of Stard7 is associated with exaggerated allergic disease.
(A) Schematic of allergen sensitization and challenge in WT and Stard7+/− littermates in the C57BL/6J background. Mice were intraperitoneally (i.p.) sensitized with 50 μg of OVA (alum) on days 0 and 7, followed by intratracheal (i.t.) challenge with 10 μg of OVA on days 18 through 21. Control mice were sensitized with PBS (alum) or OVA (alum) and challenged with PBS. Mice were analyzed on day 22. (B) H&E staining of lung tissue following sensitization and challenge. n = 6–8 mice/group. Scale bars = 50 μm. (C) Total (left panel) and differential (right panel) BALF cell counts in mice following sensitization and challenge. n = 6–8 mice/group. Mac, macrophages; Eosin, eosinophils; Neu, neutrophils; Lym, lymphocytes.