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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 May 13;194(12):5926–5936. doi: 10.4049/jimmunol.1500385

Figure 3. Number of splenic hapten-specific B cells prior to immunization correlated to vaccine efficacy.

Figure 3

Pre-vaccination B cell analysis by spleen biopsy was performed in a naïve cohort of mice and then randomly immunized with either KLH or 6OXY-KLH (n=12 each group). Data shown are the number of 6OXY-specific B cells per spleen biopsy sample from individual mice. In mice immunized with 6OXY-KLH: A) 6OXY-specific serum IgG titers vs serum oxycodone, B) 6OXY-specific IgMhigh B cells, and C) 6OXY-specific swIg B cells vs 6OXY-specific serum IgG titers. In mice immunized with 6OXY-KLH or KLH: D) 6OXY-specific serum IgG titers vs brain oxycodone, E) 6OXY-specific IgMhigh B cells and F) 6OXY-specific swIg B cells vs brain oxycodone. Each group is a composite of three independent experiments with n=12 each immunization group.