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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 May 6;194(12):5736–5742. doi: 10.4049/jimmunol.1401986

Figure 4. Increased inflammatory cytokines in serum and colon of Tipe−/− mice.

Figure 4

WT and Tipe−/− (n=5) mice were treated with DSS water for five days, followed by regular drinking water for two days. Mice were then sacrificed, and their blood was collected retro-orbitually. Colons were harvested and ½ of them were homogenized in lysis buffer (weight/volume = 1/10). The cytokines in sera and colon homogenates were measured by ELISA. (A) IL-6 and IL-17 -in sera. (B) IL-6, IL-17, and IL-1β in colon homogenates. (C) Total RNA was isolated from the other half of the colon using Trizol reagent. The levels of IL-17, IL-1β, and CXCL2 mRNAs were examined using quantitative real-time PCR. Data shown are representatives of two experiments. *P<0.05; **P<0.01; *** P<0.001.