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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 May 13;194(12):5999–6010. doi: 10.4049/jimmunol.1402719

Figure 9.

Figure 9

In vitro stimulation with an SSA1-expressing cryptococcus is sufficient to decrease M1 and increase M2 activation markers in bone marrow derived macrophages. mRNA was extracted from bone marrow-derived macrophages stimulated with live H99 or Δssa1 C. neo for 24 hours and qPCR was performed for M1 activation marker iNOS (A) and M2 activation markers Arg1 (A), CD206 (B) and Gal3 (B). iNOS/Arg1 expression ratio (A) was also prepared. Note the increase in iNOS expression and iNOS/Arg1 ratio and the decreases in Arg1, CD206, and Gal3 expression in the Δssa1-infected macrophages relative to the H99-infected macrophages. N=6–9 from three separate experiments. * p < 0.05, ** p < 0.01 using Student’s t-test.