FIGURE 4. Knockdown of MCPIP inhibited IL-4-induced ROS, ER stress and autophagy.

The peritoneal macrophages isolated from C57BL/6 mice were transfected with non-targeted siRNA (NT) or siRNA against MCPIP for 24 hr, and then treated with 20ng/ml of IL-4 for 4 hr. IL-4-induced production of ROS was measured with DHR123 (A), ER stress was measured by expression of IRE-1 and GRP78 (B, C, D) and autophagy was measured by expression of Beclin-1 transcript (E) and protein (F), autophagosome staining (G) and LC3II:LC3I ratio (H). ROS production, ER stress and autophagy were inhibited by siRNA against MCPIP, but not by nontargeted siRNA. ^*P < 0.05 vs Control, ^#P <0.05 vs NT siRNA. Experiments were repeated three times. Six different fields containing at least 200 cells were analyzed for ROS production and autophagosome staining.