FIGURE 7. Macrophages deficient in MCPIP failed to undergo M2 polarization and macrophages expressing MCPIP promoted M2 polarization.

Peritoneal macrophages from wild type and myelo-KO mice were treated with IL-4 for the indicated times and expression of MCPIP transcript was measured by qRT-PCR (A) and protein by immunoblot (B). Expression of M2 markers Arg1, YM1 and FIZZR1 in the IL-4 treated macrophages was measured by qRT-PCR (C, D, E). Phagocytosis by macrophages from myelo-KO and WT mice was measured by Zymosan internalization assay (F). Macrophages from WT and myelo-MCPIP mice were treated with LPS for the indicated periods and expression of M1 markers TNF-α, IL-1β, and iNOS was measured by qRT-PCR (G, H, I). Expression of M2 macrophage markers Arg1, YM1, and FIZZ1 in myelo-MCPIP and WT macrophages was measured by qRT-PCR (J, K, L). P < 0.05 compared with macrophages isolated from wild-type mice (n = 3 per each genotype).