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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Anal Biochem. 2015 Apr 29;482:1–6. doi: 10.1016/j.ab.2015.04.025

Figure 1. The chemistry does not alter the fluorescence of GFP-C/EBP.

Figure 1

When 100 μl of 8.5 μg/ml (~200 nM) purified GFP-C/EBP was reacted with 20 μl of 50 mM NaIO4 (or water) and 20 μl Na2S2O5 (or water) and then diluted with 400 μl of buffer GS (50 mM Tris, pH 8, 5% glycerol, 1mM EDTA, 1 mM 2-mercaptoethanol), the fluorescence (λex = 398 nm, λem = 512 nm) is not altered. Shown are the average and standard deviation for duplicates. In other experiments (data not shown), the excitation and emission spectra were also unaltered.