Figure 7. Other E-box binding transcriptions are also purified by affinity chromatography with some differences.

500 μg HEK293 nuclear extract was prepared for affinity chromatography by dilution into buffer TE0.1. This was applied to a 0.1 ml column prepared from 20 pmoles annealed SJ9/SJ11 duplex oligonucleotide by aldehyde coupling. The flow-through (FT) was collected and the column was washed with 0.5 ml of TE0.1 buffer and then eluted with 0.5 ml of TE1.5. The conditions otherwise are the same as in Fig. 6.