. Author manuscript; available in PMC: 2016 Jul 1.

Published in final edited form as: J Immunol Methods. 2015 Apr 9;422:111–117. doi: 10.1016/j.jim.2015.04.001

Table 1. Characteristics of murine and chimeric mAbs used in this study.

mAb	Species^a	Isotype	Target	Agglutination (μg/ml)^b	Vibriocidal^c
2D6	mouse	IgA	O-PS	18	no
	chimeric	IgG₁		nd	yes
ZAC-3	mouse	IgA	Lipid A/core	0.25	no
	chimeric	IgG₁		nd	yes

Chimeric antibodies were composed of a murine variable region grafted onto a human IgG₁ constant region.

Lowest antibody concentration that induced visible agglutination of V. cholerae O395 after 2 hr incubation at 37°C, nd: none detected.

Late exponential phase V. cholerae O395 was pre-mixed with guinea pig serum and incubated in LB media containing 100 μg/ml of antibody. Bacterial samples were diluted and plated for CFUs. Vibriocidal activity was defined as ≥1 log decrease in CFU compared to control treatment.