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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 Apr 29;194(12):5872–5884. doi: 10.4049/jimmunol.1402154

Figure 2.

Figure 2

Deficiency for autophagy genes inhibited iNKT cell maturation. (A,C) Thymus and spleen cells from eight-to-nine-week old mice were analyzed for iNKT cell phenotype. Representative flow cytometry plots from one of four experiments showing gated iNKT cells from Atg5f/f CD4-Cre (A) or Atg7f/f CD4-Cre (C) mice and controls lacking the Cre transgene. Absolute cell numbers of thymic iNKT cells at different stages were shown in (B, Atg5f/f CD4-Cre and D, Atg7f/f CD4-Cre). The analysis of all stages was carried out simultaneously, but because stages 0 and 1 have much fewer cells, for visual clarity their numbers are plotted on a separate graph (left). *p<0.0005, **p<0.0001, n≥10. Error bars are SD.