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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 Apr 29;194(12):5872–5884. doi: 10.4049/jimmunol.1402154

Figure 4.

Figure 4

Atg5 or Atg7 deficiency did not cause significant DP thymocyte death and skewed TCR α rearrangements in thymocytes. (A-C) Thymocytes were cultured overnight and cells undergoing apoptosis and dead cells were identified, shown here as flow cytometry analyses (A) and the geometric MFI results indicating cells undergoing apoptosis (B) and dead cells (C). Data in (A) are representative of at least four independent experiments. (B and C) n≥5. Error bars are SD. (D). Equivalent TCR Vα14-Jα18 rearrangements were observed in Atg5 or Atg7 deficient DP thymocytes. cDNA reverse transcribed from total RNA of DP thymocytes depleted of αGalCer-CD1d tetramer+ cells was used as template and titrated at serial dilutions (200 ng, 60 ng and 20 ng) for semi-quantitative PCR. Representative data are from three separate experiments.