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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: J Immunol. 2015 Apr 29;194(12):5872–5884. doi: 10.4049/jimmunol.1402154

Figure 9.

Figure 9

Unbalanced proliferative and apoptotic signaling in Atg5 deficient iNKT cells. Thymic iNKT cells were analyzed ex vivo for activation of mTOR signaling pathway. Phosphorylation of mTORC1 substrate, 4E–BP1 (pT37/pT46) (A), and phosphorylation of mTORC2 substrate, AKT(pS473) (B), were analyzed. (C) Phosphorylation of AKT at T308 was measured to evaluate the PI3K/PDK1 signaling pathway. Both Cre- and Cre+ were pooled from at least four samples. Shown here is the representative flow cytometry analysis of three independent experiments. Filled histogram: isotype control; solid line: wild type mice; dotted line: Atg5f/f CD4-Cre mice. *p<0.05, **p<0.01, ***p<0.005, ****p<0.0005, n≥4. Error bars are SD.