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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 13;75(7):1376–1387. doi: 10.1158/0008-5472.CAN-14-1931

Figure 2. MMAE increases IR induced DNA double strand breaks in a schedule and dose dependent manner.

Figure 2

A) HCT-116 cells were treated with 5 nM MMAE for 2, 4, or 24 hours followed by 6 Gy. Accumulation of cyclins was assessed by immunoblotting at time of irradiation. Comet tail length was measured using neutral comet assay 15 minutes post IR. Data are plotted as mean comet tail length ± SEM with non-irradiated comet tail length subtracted. B, C) HCT-116 cells were treated with 0, 1, or 5 nM MMAE for 24 hrs and then irradiated with 6 Gy. Comet tail length was measured using neutral comet assay. Data are plotted as mean comet tail length ± SEM. Representative images from comet tail assay are shown for MMAE dose of 5 nM. *P<0.01, **P<0.0001