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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 13;75(7):1376–1387. doi: 10.1158/0008-5472.CAN-14-1931

Figure 5. Activatable cell penetrating peptides are cleaved in irradiated tumor microenvironments.

Figure 5

A) Orthotopic pancreatic adenocarcinoma PDX were harvested and zymography gels used to assess gelatinase activity, lysates. For each PDX, lysates were run in duplicate (lanes A and B). B-D) HCT-116 or PANC-1 tumor xenografts were grown in both the left and right hindlimbs of nude mice. The right tumor was irradiated with 6 Gy and the left tumor was shielded to block out >95% of the IR dose. B) Zymography gels were used to asses MMP activity in non-irradiated and irradiated tumors. C) β3 integrin expression by IHC in non-irradiated and irradiated PANC-1 tumors D) One day post IR, ratiometric ACPP was intravenously injected and Cy5:Cy7 emission ratio measured (pseudocolor scale at far right) by whole animal imaging with tumors in situ and after tumor excision.