Table 2.

Transcriptional expression levels of copper regulatory proteins in SVZ and choroid plexus of young, adult and old rats.

		SVZ (ΔΔ Ct value)			Choroid plexus (ΔΔ Ct value)
Category	Gene	Young rats	Adult rats	Old rats	Young rats	Adult rats	Old rats
Transporters	Ctr1	0.0145 ± 0.0007	0.0105 ± 0.0009aa	0.0095 ± 0.0010aa	0.4364 ± 0.0817**	0.5291 ± 0.0568aa##	0.5904 ± 0.0705aabΔΔ
	Dmt1	0.0108 ± 0.0017	0.0095 ± 0.0028	0.0126 ± 0.0024b	0.0294 ± 0.0033**	0.0350 ± 0.0037aa##	0.0440 ± 0.0041aabbΔΔ
	Atp7a	0.0016 ± 0.0003	0.0016 ± 0.0003	0.0017 ± 0.0003	0.0152 ± 0.0040**	0.0131 ± 0.0017a##	0.0149 ± 0.0021ΔΔ
	Atp7b	0.0013 ± 0.0001	0.0009 ± 0.0003	0.0015 ± 0.0004bb	0.0010 ± 0.0004	0.0008 ± 0.0001	0.0009 ± 0.0002
Chaperones	Cox17	0.0547 ± 0.0109	0.0531 ± 0.0081	0.0663 ± 0.0297	0.1052 ± 0.0244**	0.1189 ± 0.0082##	0.1244 ± 0.0210aΔΔ
	Atox1	0.0024 ± 0.0024	0.0027 ± 0.0011	0.0027 ± 0.0013	0.0226 ± 0.0046**	0.0125 ± 0.0027aa##	0.0118 ± 0.0034aabbΔΔ
	Ccs	0.0207 ± 0.0055	0.0244 ± 0.0058	0.0323 ± 0.0125aa	0.0555 ± 0.0105**	0.0634 ± 0.0050##	0.0816 ± 0.0082aabbΔΔ
Binding Proteins	Mt1a	0.0630 ± 0.0125	0.0406 ± 0.0138aa	0.0985 ± 0.0282aabb	0.0560 ± 0.0131	0.0319 ± 0.0053aa	0.0483 ± 0.0126bbΔΔ
	Mt2a	0.2289 ± 0.0461	0.1288 ± 0.0515aa	0.2648 ± 0.1049bb	0.0247 ± 0.0159**	0.0119 ± 0.0032##	0.0189 ± 0.0133ΔΔ
	Mt3	0.2330 ± 0.0618	0.2970 ± 0.1008	0.8869 ± 0.3864aabb	0.1349 ± 0.0715**	0.0473 ± 0.0059aa##	0.1414 ± 0.0237bbΔΔ

Note: Data represents mean ± S.D., n = 6. SVZ, subventricular zone. Comparison between SVZ and choroid plexus with the same age: **p < 0.01, as compared with the SVZ of young rats; ^##p < 0.01, as compared with the SVZ of adult rats; ΔΔ: p < 0.01, as compared with the SVZ of old rats. Comparison among different age groups within SVZ or choroid plexus tissues: a: p < 0.05, aa: p < 0.01, as compared with young rats; b: p < 0.05, bb: p < 0.01, as compared with adult rats.