Category	Gene name	Primer sequence
Transporters	Ctr1	Forward	5′-TCG GCC TCA CAC TCC CAC GA-3′
		Reverse	5′-CGA AGC AGA CCC TCT CGG GC-3′
	Dmt1	Forward	5′-TCG CAG GCG GCA TCT TGG TC-3′
		Reverse	5′-TAC CGA GCG CCC ACA GTC CA-3′
	Atp7a	Forward	5′-CTT GTA GAG GAG GCA CAG AC-3′
		Reverse	5′-GGT AAC AAT GGA AAC CAA GA-3′
	Atp7b	Forward	5′-AAT CCA GGA CTG TCC GTT CTA A-3′
		Reverse	5′-CAC TTG CTC CTC TCT GAG GAT T-3′
Chaperons	Cox17	Forward	5′-CTG AGT TTT GGG AGC TTT GC-3′
		Reverse	5′-AGG GCT TCA GAG GCT TCT TC-3′
	Ccs	Forward	5′-TCA CAG GGA ATT CTG GGA AG-3′
		Reverse	5′-GGA GGC TCT GTT CAG AGG TG-3′
	Atox1	Forward	5′-CTC AAC AAA ACA GGA AAA GC-3′
		Reverse	5′-GAT CAA CAG TCT GCC TCT TC-3′
Binding Proteins	Mt1a	Forward	5′-GCC TTC TTG TCG CTT ACA CC-3′
		Reverse	5′-AGG AGC AGC AGC TCT TCT TG-3′
	Mt2a	Forward	5′-ACA GAT GGA TCC TGC TCC TG-3′
		Reverse	5′-GAG AAC CGG TCA GGG TTG TA-3′
	Mt3	Forward	5′-CCC TGC AGG ATG TGA GAA GT-3′
		Reverse	5′-TTT GCT GTG CAT GGG ATT TA-3′
Reference Gene	Actb	Forward	5′-AGC CAT GTA CGT AGC CAT CC-3′
		Reverse	5′-CTC TCA GCT GTG GTG GTG AA-3′