Figure 1. The effect of hyphae on IL-8 production and mRNA expression.

A: The RNAs from cells treated with different concentrations of A. fumigatus hyphae (5×10⁵, 5×10⁶ and 5×10⁷/mL) were harvested and used for RT-PCR; B: Cells were stimulated with A. fumigatus hyphae at 37°C. The supernatants were collected and assayed for IL-8 by ELISA. The data represented the mean±SEM of three separate experiments. ^aP<0.05 versus control alone; ^bP<0.01 versus control alone.