Figure 3. Engagement of Dectin-1 activates Ca²⁺-dependent PKC with stimulation of A. fumigates hyphae in HCECs.

A, B and C: HCECs were treated with 5×10⁶/mL A. fumigatus hyphae for various times. The activation status of PKCα, PKCβI and PKCβII were determined by Western blot (WB), respectively. D, E and F: HCECs were preincubated for 30min with Dectin-1 inhibitor laminarin, followed by treatment with hyphae for 30min. The activation of PKCα, PKCβI and PKCβII was assayed by WB. Data shown were representative of more than three independent sets of experiments. ^aP<0.05.