Fig. 1.

MLE interacts with Hrb87F and Pep. A, Aliquots from samples used in Fig. 2A were analyzed by Western blot with anti-Pep and anti-Hrb87F antibodies. Both proteins bind MLE regardless of the presence or absence of the MSL complex. Treatment with RNase A completely abrogates the binding. MLE-FLAG is stained with Coomassie. B, qRT-PCR showing the effect of an mle null mutation on the transcription of the ecdysone-responsive genes (light gray) versus ecdysone-unrelated genes (dark gray).