Fig. 2.

MLE interacts with members of the NuRD complex. A, MLE-FLAG immunoprecipitation from S2 cells extracts. MLE binds the MEP-1 and Mi-2 subunits of the NuRD complex; this binding is not affected by the presence of the MSL complex but is severely affected by RNase treatment. Mock samples are S2 cell extracts containing FLAG peptide. MLE-FLAG is stained with Coomassie. B, MEP-1 immunoprecipitation from S2 cell extracts. MEP-1 binds endogenous MLE whereas MSL1 does not appear to be present in the precipitate. Mi-2 was used as a positive control for the immunoprecipitation. Mock IP is performed with generic IgG. C, Mi2 immunoprecipitation from S2 cell extracts. Mi-2 binds its partner MEP-1 and MLE but not MSL1. MSL1 is reduced in the MSL2 knockdown samples because it is largely unstable without MSL2. Mock IP is performed with generic IgG. D, MLE-FLAG immunoprecipitation from S2 cell extracts. p66 binding to MLE does not require the MSL complex and is partially affected by RNase treatment. Mock samples are S2 cell extracts containing FLAG peptide. MLE-FLAG is stained with Coomassie. E, p66 immunoprecipitation from S2 cell extracts. p66 specifically interacts with MLE and with its known partner Mi-2. Mock IP is performed with generic IgG.