Table II. Down-regulated proteins (fold change < 0.5) in HuH-7R cells.
| Accession number | Description | Symbol | Coverage (%) | Match peptides | H/L ratio | Mascot scores | Coverage (%) | Match peptides | 116/114 ratioa | Mascot scores | Metastasis |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Q15493 | Regucalcin | RGN | 5.69 | 1 | 0.034 | 144.88 | 15.72 | 4 | 0.096 | 81.07 | |
| P35527 | Keratin, type I cytoskeletal 9 | KRT9 | 28.41 | 9 | 0.045 | 897.22 | 7.38 | 6 | 0.196 | 49.60 | |
| Q96DC8 | Enoyl-CoA hydratase domain-containing protein 3, mitochondrial | ECHDC3 | 13.86 | 2 | 0.071 | 129.67 | 8.25 | 2 | 0.195 | 36.11 | |
| P28332 | Alcohol dehydrogenase 6 | ADH6 | 6.52 | 1 | 0.072 | 56.71 | 14.95 | 6 | 0.056 | 103.45 | |
| P55809 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial | OXCT1 | 11.92 | 3 | 0.081 | 99.65 | 6.92 | 3 | 0.282 | 53.14 | |
| P55157 | Microsomal triglyceride transfer protein large subunit | MTTP | 26.06 | 16 | 0.101 | 684.92 | 25.62 | 20 | 0.103 | 527.12 | |
| Q13423 | NAD(P) transhydrogenase, mitochondrial | NNT | 15.10 | 10 | 0.104 | 916.85 | 10.77 | 11 | 0.138 | 277.28 | |
| P48728 | Aminomethyltransferase, mitochondrial | AMT | 3.47 | 1 | 0.111 | 91.44 | 1.99 | 1 | 0.109 | 22.01 | |
| P21397 | Amine oxidase [flavin-containing] A | MAOA | 11.20 | 4 | 0.115 | 181.98 | 9.11 | 6 | 0.093 | 73.67 | |
| P32189 | Glycerol kinase | GK | 20.21 | 9 | 0.129 | 550.13 | 15.56 | 9 | 0.165 | 84.68 | |
| P23141 | Liver carboxylesterase 1 | CES1 | 53.44 | 24 | 0.152 | 5133.25 | 24.34 | 12 | 0.169 | 622.79 | |
| Q13228 | Selenium-binding protein 1 | SELENBP1 | 2.75 | 1 | 0.153 | 48.25 | 11.02 | 4 | 0.127 | 154.39 | |
| P00352 | Retinal dehydrogenase 1 | ALDH1A1 | 60.68 | 25 | 0.159 | 6964.85 | 31.14 | 14 | 0.318 | 253.37 | |
| P15144 | Aminopeptidase N | ANPEP | 2.17 | 2 | 0.160 | 75.51 | 10.13 | 10 | 0.235 | 190.45 | |
| P00367 | Glutamate dehydrogenase 1, mitochondrial | GLUD1 | 39.43 | 17 | 0.164 | 2869.48 | 35.48 | 20 | 0.064 | 1497.60 | |
| Q96CM8 | Acyl-CoA synthetase family member 2, mitochondrial | ACSF2 | 8.62 | 3 | 0.168 | 297.04 | 6.34 | 4 | 0.417 | 36.70 | |
| P00966 | Argininosuccinate synthase | ASS1 | 31.80 | 7 | 0.168 | 262.42 | 4.85 | 3 | 0.276 | 23.84 | |
| Q86TX2 | Acyl-coenzyme A thioesterase 1 | ACOT1 | 40.38 | 10 | 0.170 | 874.14 | 15.91 | 7 | 0.297 | 188.53 | |
| O43175 | D-3-phosphoglycerate dehydrogenase | PHGDH | 49.72 | 17 | 0.171 | 1551.32 | 18.57 | 9 | 0.126 | 432.92 | |
| P11498 | Pyruvate carboxylase, mitochondrial | PC | 35.40 | 25 | 0.172 | 3147.43 | 15.11 | 14 | 0.068 | 220.94 | |
| P42330 | Aldo-keto reductase family 1 member C3 | AKR1C3 | 57.28 | 14 | 0.174 | 4764.61 | 21.05 | 9 | 0.046 | 332.48 | |
| O60701 | UDP-glucose 6-dehydrogenase | UGDH | 60.93 | 23 | 0.183 | 4150.41 | 15.99 | 7 | 0.133 | 153.19 | |
| P49888 | Estrogen sulfotransferase | SULT1E1 | 7.14 | 1 | 0.185 | 45.56 | 5.44 | 2 | 0.209 | 81.80 | |
| P05091 | Aldehyde dehydrogenase, mitochondrial | ALDH2 | 26.11 | 10 | 0.191 | 997.67 | 31.72 | 15 | 0.204 | 565.98 | |
| P32119 | Peroxiredoxin-2 | PRDX2 | 35.86 | 7 | 0.203 | 661.98 | 33.33 | 8 | 0.149 | 453.16 | |
| Q02252 | Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial | ALDH6A1 | 19.44 | 8 | 0.205 | 260.38 | 23.36 | 10 | 0.303 | 219.25 | |
| Q9H3G5 | Probable serine carboxypeptidase CPVL | CPVL | 13.24 | 5 | 0.211 | 319.41 | 4.20 | 3 | 0.272 | 45.97 | |
| P50225 | Sulfotransferase 1A1 | SULT1A1 | 28.81 | 5 | 0.218 | 439.29 | 20.34 | 8 | 0.144 | 37.04 | |
| O75874 | Isocitrate dehydrogenase [NADP] cytoplasmic | IDH1 | 45.17 | 14 | 0.220 | 2770.34 | 33.57 | 14 | 0.257 | 598.48 | |
| Q9Y365 | PCTP-like protein | STARD10 | 10.31 | 2 | 0.220 | 215.94 | 9.62 | 2 | 0.092 | 48.96 | |
| Q96C23 | Aldose 1-epimerase | GALM | 19.59 | 4 | 0.221 | 347.19 | 14.04 | 4 | 0.089 | 181.34 | |
| Q9ULC5 | Long-chain-fatty-acid–CoA ligase 5 | ACSL5 | 26.94 | 13 | 0.225 | 1026.06 | 18.89 | 13 | 0.049 | 263.76 | |
| P78330 | Phosphoserine phosphatase | PSPH | 32.44 | 5 | 0.233 | 537.24 | 25.33 | 5 | 0.215 | 191.88 | |
| P45954 | Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial | ACADSB | 19.91 | 5 | 0.236 | 489.46 | 23.61 | 8 | 0.242 | 146.90 | |
| P16401 | Histone H1.5 | HIST1H1B | 10.18 | 3 | 0.238 | 119.03 | 31.86 | 9 | 0.245 | 675.56 | |
| Q6P587 | Acylpyruvase FAHD1, mitochondrial | FAHD1 | 33.48 | 4 | 0.250 | 322.07 | 3.57 | 1 | 0.446 | 31.41 | |
| Q08426 | Peroxisomal bifunctional enzyme | EHHADH | 7.19 | 4 | 0.260 | 185.39 | 15.35 | 11 | 0.031 | 264.25 | |
| P09455 | Retinol-binding protein 1 | RBP1 | 32.59 | 4 | 0.260 | 216.97 | 24.44 | 3 | 0.366 | 177.82 | |
| P23378 | Glycine dehydrogenase [decarboxylating], mitochondrial | GLDC | 15.49 | 10 | 0.261 | 696.72 | 7.06 | 7 | 0.013 | 149.08 | |
| P56199 | Integrin alpha-1 | ITGA1 | 4.58 | 4 | 0.272 | 131.10 | 4.92 | 8 | 0.429 | 28.65 | |
| Q00796 | Sorbitol dehydrogenase | SORD | 34.73 | 8 | 0.291 | 1004.66 | 21.85 | 6 | 0.403 | 101.08 | |
| Q9Y617 | Phosphoserine aminotransferase | PSAT1 | 30.27 | 9 | 0.292 | 935.75 | 29.73 | 12 | 0.127 | 224.59 | |
| P51812 | Ribosomal protein S6 kinase alpha-3 | RPS6KA3 | 31.22 | 18 | 0.312 | 1508.24 | 21.22 | 17 | 0.089 | 407.58 | |
| Q08623 | Pseudouridine-5′-monophosphatase | HDHD1 | 12.28 | 3 | 0.315 | 260.60 | 8.33 | 2 | 0.489 | 31.00 | |
| P13804 | Electron transfer flavoprotein subunit alpha, mitochondrial | ETFA | 50.15 | 10 | 0.320 | 1796.72 | 25.23 | 6 | 0.369 | 149.78 | |
| P15374 | Ubiquitin carboxyl-terminal hydrolase isozyme L3 | UCHL3 | 31.30 | 5 | 0.323 | 603.32 | 19.57 | 4 | 0.293 | 68.79 | |
| P30405 | Peptidyl-prolyl cis-trans isomerase F, mitochondrial | PPIF | 27.05 | 2 | 0.329 | 366.13 | 17.39 | 5 | 0.243 | 85.43 | |
| Q2PPJ7 | Ral GTPase-activating protein subunit alpha-2 | RALGAPA2 | 2.35 | 3 | 0.336 | 122.83 | 1.71 | 4 | 0.044 | 58.45 | √ |
| Q9P015 | 39S ribosomal protein L15, mitochondrial | MRPL15 | 16.89 | 3 | 0.338 | 379.63 | 15.20 | 5 | 0.407 | 42.49 | |
| Q5T6V5 | UPF0553 protein C9orf64 | C9orf64 | 26.69 | 7 | 0.348 | 623.85 | 12.61 | 4 | 0.220 | 64.10 | |
| P38117 | Electron transfer flavoprotein subunit beta | ETFB | 45.49 | 12 | 0.359 | 1048.97 | 43.53 | 12 | 0.281 | 250.59 | |
| Q9NRF8 | CTP synthase 2 | CTPS2 | 19.45 | 8 | 0.364 | 819.80 | 8.53 | 5 | 0.017 | 43.45 | |
| Q15125 | 3-beta-hydroxysteroid-Delta(8), Delta(7)-isomerase | EBP | 6.96 | 1 | 0.365 | 106.53 | 11.30 | 2 | 0.371 | 38.10 | |
| Q9UIJ7 | GTP:AMP phosphotransferase, mitochondrial | AK3 | 20.70 | 3 | 0.368 | 496.73 | 32.16 | 7 | 0.446 | 123.54 | |
| O15228 | Dihydroxyacetone phosphate acyltransferase | GNPAT | 13.68 | 7 | 0.379 | 533.17 | 4.41 | 3 | 0.282 | 38.20 | |
| P35754 | Glutaredoxin-1 | GLRX | 23.58 | 3 | 0.388 | 190.41 | 10.38 | 1 | 0.097 | 35.60 | |
| P48506 | Glutamate–cysteine ligase catalytic subunit | GCLC | 7.69 | 5 | 0.390 | 369.81 | 4.55 | 3 | 0.394 | 55.76 | |
| Q9HC35 | Echinoderm microtubule-associated protein-like 4 | EML4 | 12.23 | 9 | 0.404 | 667.70 | 10.70 | 10 | 0.229 | 198.44 | |
| O14936 | Peripheral plasma membrane protein CASK | CASK | 7.02 | 4 | 0.407 | 369.08 | 7.13 | 9 | 0.350 | 39.70 | |
| P51690 | Arylsulfatase E | ARSE | 8.49 | 3 | 0.407 | 185.17 | 7.64 | 4 | 0.253 | 111.16 | |
| P09417 | Dihydropteridine reductase | QDPR | 43.85 | 6 | 0.411 | 705.23 | 31.97 | 5 | 0.377 | 130.89 | |
| P11766 | Alcohol dehydrogenase class-3 | ADH5 | 34.49 | 7 | 0.422 | 846.22 | 11.23 | 5 | 0.353 | 128.03 | |
| P21291 | Cysteine and glycine-rich protein 1 | CSRP1 | 19.17 | 2 | 0.423 | 111.06 | 11.92 | 2 | 0.430 | 58.02 | |
| O94832 | Unconventional myosin-Id | MYO1D | 2.49 | 2 | 0.423 | 125.92 | 6.06 | 8 | 0.416 | 55.25 | |
| Q9BRX8 | Redox-regulatory protein FAM213A | FAM213A | 16.59 | 3 | 0.432 | 292.31 | 26.64 | 6 | 0.294 | 144.68 | |
| Q9NQ94 | APOBEC1 complementation factor | A1CF | 8.92 | 4 | 0.442 | 316.45 | 6.06 | 4 | 0.373 | 29.11 | |
| P28288 | ATP-binding cassette sub-family D member 3 | ABCD3 | 21.70 | 12 | 0.447 | 1180.54 | 6.07 | 4 | 0.196 | 78.63 | |
| P25325 | 3-mercaptopyruvate sulfurtransferase | MPST | 22.90 | 4 | 0.447 | 521.80 | 19.53 | 5 | 0.365 | 107.95 | |
| Q13126 | S-methyl-5′-thioadenosine phosphorylase | MTAP | 25.09 | 4 | 0.448 | 520.92 | 6.01 | 2 | 0.371 | 36.03 | |
| Q14571 | Inositol 1,4,5-trisphosphate receptor type 2 | ITPR2 | 1.37 | 4 | 0.456 | 187.79 | 4.85 | 14 | 0.490 | 68.61 | |
| Q9H0U6 | 39S ribosomal protein L18, mitochondrial | MRPL18 | 5.00 | 1 | 0.457 | 50.52 | 7.22 | 1 | 0.459 | 48.78 | |
| P27144 | Adenylate kinase isoenzyme 4, mitochondrial | AK4 | 11.66 | 2 | 0.464 | 361.78 | 24.22 | 5 | 0.144 | 73.66 | |
| Q9BRF8 | Calcineurin-like phosphoesterase domain-containing protein 1 | CPPED1 | 12.42 | 3 | 0.478 | 274.46 | 6.37 | 2 | 0.177 | 86.52 | |
| Q8NBQ5 | Estradiol 17-beta-dehydrogenase 11 | HSD17B11 | 23.67 | 5 | 0.486 | 371.70 | 15.00 | 5 | 0.420 | 148.64 | |
| O94905 | Erlin-2 | ERLIN2 | 27.73 | 8 | 0.492 | 823.15 | 34.51 | 10 | 0.480 | 246.01 |
a 117/115 ratios are similar to 116/114 ratios. In this table, we only show the 116/114 ratios in iTRAQ experiment.