Fig. 1.
Representative hits of the kinome esiRNA screen. Average normalized fluorescence intensity (ΔFIavg) values of ΔF508-CFTR cells (coexpressing eYFP(H148Q/I152L) that were transfected with esiRNA directed toward A, FGFR1, B. RIPK4, C, MET, D, SHPK, E, MAP3K13, F, BRAF, G, DUSP22, H, CDK10, I, IPMK, or luciferase (nonsilencing control), and grown at 37 °C. After 72 h ΔF508-CFTR cells were stimulated with FIG (25 μm Forskolin, 45 μm IBMX, and 50 μm Genistein) and quenching of YFP fluorescence due to of Cl−/I− exchange was quantified by Cellomics KSR Reader (70–300 cells per well). J, Quantitation of rescue (difference in average fluorescence intensity ΔFIavg) of ΔF508-CFTR at 24 s after adding iodide solution from three independent experiments (a single field per well, 70–300 cells per field). Data are mean ± S.E.