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. 2015 Mar 29;14(6):1569–1583. doi: 10.1074/mcp.M114.046375

Fig. 3.

Fig. 3.

Effect of shRNA-mediated knockdown of the hit genes on surface expression of ΔF508-CFTR. 293MSR-GT cells stably expressing ΔF508-CFTR (bearing a 3HA tag at the ectodomain) were transfected with shRNA for the analyzed genes or nonsilencing control (as indicated), grown at 37 °C for 48 h, selected on puromycin, and quantitation of surface expression of ΔF508-CFTR was carried out by ELISA assay. SU5402 represents cells treated with SU5402 and serves as a positive control. Data are mean ± S.E. from three independent experiments.