Fig. 6.

Chaperone expression analysis following FGFR inhibition by SU5402. A, Response (Isc) of ΔF508/ΔF508-CFTR HBE cells obtained from two separate patients to SU5402 analyzed by Ussing chambers, highlighting the response of the Responder and Poor responder to 48 h treatment with 1 μm or 10 μm SU5402. CFTR currents were stimulated with FIG (25 μm Forskolin, 25 μm IBMX, and 50 μm Genistein) and after the indicated time inhibited using 15 μm GlyH-101 (Gly). Inset: summary of three separate experiments (mean ± S.E.). B, Heat map of chaperone expression profile following 48 h treatment of ΔF508/ΔF508-CFTR HBE cells (from the Responder and Poor responder) with 1 μm or 10 μm SU5402, as indicated. Data are average of three separate arrays (normalized to DMSO control).