Fig. 7.

Validation of chaperone hits. ELISA analysis of cell surface expression of ΔF508-CFTR (relative to luciferase control) expressed in 293-GT cells following, A, overexpression of the top chaperones that exhibited elevated expression following SU5402 treatment in the heat map (Fig. 6), or B, shRNA-mediated knockdown of chaperones that exhibited reduced expression in the heat map following SU5402 treatment. The extent of knockdown (two clones per gene) is shown in Supplemental Table S3. Data in A and B are mean ± S.E., n = three independent experiments.