Table II. Validation of hits with shRNA using the halide-exchange assay Hits were validated by functional assay (Cellomics). Rescue by the best shRNA clone and th.e corresponding knockdown level are shown.
| Gene name | Validation by functional assay (Cellomics) |
Knockdown level (%) | |
|---|---|---|---|
| Analyzed shRNA clones | Rescue by the best shRNA clone (ΔFIavg) | ||
| FGFR1 | 10 | 0.30 | 94 |
| RIPK4 | 2 | 0.22 | 26 |
| MET | 17 | 0.16 | 61 |
| SHPK | 3 | 0.15 | 52 |
| MAP3K13 | 4 | 0.15 | 65 |
| BRAF | 7 | 0.15 | 34 |
| DUSP22 | 7 | 0.15 | 71 |
| CDK10 | 6 | 0.14 | 87 |
| IPMK | 10 | 0.13 | 63 |
| RPS6KC1 | 2 | 0.13 | 96 |
| PRKAR2B | 2 | 0.12 | 40 |
| PANK4 | 15 | 0.12 | 80 |
| SOCS1 | 5 | 0.12 | N/A |
| PCK2 | 4 | 0.12 | 78 |
| CAMK2B | 3 | 0.09 | 85 |
| DTYMK | 10 | 0.09 | 88 |
| ERN1 | 1 | 0.08 | 79 |
| CLK3 | 8 | 0.08 | 64 |
| NEK10 | 10 | 0.08 | 84 |
| PANK1 | 7 | 0.06 | 79 |